IDENTIFIKASI ASPERGILLUS SP DI RUANGAN PERPUSTAKAAN KOTA MALANG

Abstrak

Aspergillus sp adalah salah satu jenis kapang yang sering ditemukan di lingkungan yang kaya bahan organik keberadaannya di ruangan perpustakaan dapat menjadi sumber kontaminasi mikroba yang berpotensi membahayakan kesehatan pengunjung. Tujuan Penelitian ini bertujuan mengidentifikasi tingkat polusi Aspergillus sp di ruangan Perpustakaan Kota Malang. Penelitian Maret–April 2024 dengan Random Sampling, tahapan pada penelitian meliputi isolasi jamur dengan cara perangkap udara, identifikasi makroskopis dengan memeriksa ciri-ciri koloni Aspergillus sp, identifikasi mikroskopis dengan pewarnaan Lactophenol Cotton Blue (LCB) dan pengamatan dibawah mikroskop perbesaran 40X. Hasil penelitian pada sampel R2 dan R3, (R2 ruang penitipan barang\loker, R3 ruang tamu) terdapat koloni kuning kehitaman, bulat, granular, menutupi setengah bagian atas vesikel, menandakan keberadaan Aspergillus sp. Sedangkan identifikasi mikroskopis menunjukkan adanya tangkai konidia, konidia kasar, hifa panjang bercabang, sesuai ciri Aspergillus flavus. Hasil identifikasi Aspergillus flavus di perpustakaan Kota Malang memiliki implikasi penting dalam upaya pencegahan pencemaran udara dan perlindungan kesehatan pengunjung.

Aspergillus sp, a common mold type found in environments rich in organic matter, can contaminate the air in libraries, posing potential health hazards to visitors. Objective this study aims to identify the level of Aspergillus sp pollution in the Malang City Library. Methodology the research was conducted in March-April 2024 using Random Sampling technique, including fungal isolation through air trapping. Macroscopic identification was performed by examining the characteristics of Aspergillus sp colonies, while microscopic identification was conducted using staining with Lactophenol Cotton Blue (LCB) and observation under a microscope at 40X magnification. Results in samples R2 and R3, colonies of yellowish-black color, round, granular, covering half of the vesicle's upper part, indicating the presence of Aspergillus sp, were found. Microscopic identification revealed the presence of conidial stalks, rough conidia, long branched hyphae, consistent with the characteristics of Aspergillus flavus. Conclusion the identification of Aspergillus flavus in the Malang City Library has significant implications for efforts to prevent air pollution and protect the health of visitors.